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. Author manuscript; available in PMC: 2008 Sep 23.
Published in final edited form as: Anal Chem. 2008 Aug 5;80(17):6715–6723. doi: 10.1021/ac8007994

Table 1.

Protein Solubility in Various Sample Buffers: Soluble (S), Partially Soluble (S/I), and Insoluble (I)

Protein mg/mL
Sample buffer 0.3 0.6 0.9 1.2
Urea + 0.05% TFA S S/I S/I I
Urea + 0.1 % TFA S S/I S/I I
0.05 % TFA S/I S/I I I
0.1 % TFA S/I I I I
1% acetic acid I I I I