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. Author manuscript; available in PMC: 2008 Sep 23.
Published in final edited form as: Anal Chem. 2008 Aug 5;80(17):6715–6723. doi: 10.1021/ac8007994

Table 2.

Summary of 2D Separation Methods

Type of Separation 1st Dimension Column Type 1st Dimension Mobile Phase A: 1st Dimension Mobile Phase B: Gradient Percent B:
hrProt Off-line Protein High Protein Recovery C18 0.1% TFA H2O 0.1% TFA ACN 15–55
hrProt + Urea Off-line Protein High Protein Recovery C18 0.1% TFA H2O 0.1% TFA ACN 15–55
cProt Off-line Protein Conventional 300Å C18 0.1% TFA H2O 0.1% TFA ACN 15–55
pH-RP Off-line Peptide High pH Stable C18 200mM NH4Formate pH 10 ACN 5–35
On-SCX On-line Peptide Strong Cation Exchange Ammonium Citrate 10mM Ammonium Citrate 10mM pH Buffers
Off-SCX Off-line Peptide Strong Cation Exchange 25% ACN, 5mM K2HPO4 25% ACN, 500mM K2HPO4 5–100
Gel Protein NA NA NA NA