Table 2.
Type of Separation | 1st Dimension Column Type | 1st Dimension Mobile Phase A: | 1st Dimension Mobile Phase B: | Gradient Percent B: | |
---|---|---|---|---|---|
hrProt | Off-line Protein | High Protein Recovery C18 | 0.1% TFA H2O | 0.1% TFA ACN | 15–55 |
hrProt + Urea | Off-line Protein | High Protein Recovery C18 | 0.1% TFA H2O | 0.1% TFA ACN | 15–55 |
cProt | Off-line Protein | Conventional 300Å C18 | 0.1% TFA H2O | 0.1% TFA ACN | 15–55 |
pH-RP | Off-line Peptide | High pH Stable C18 | 200mM NH4Formate pH 10 | ACN | 5–35 |
On-SCX | On-line Peptide | Strong Cation Exchange | Ammonium Citrate 10mM | Ammonium Citrate 10mM | pH Buffers |
Off-SCX | Off-line Peptide | Strong Cation Exchange | 25% ACN, 5mM K2HPO4 | 25% ACN, 500mM K2HPO4 | 5–100 |
Gel | Protein | NA | NA | NA | NA |