Table 4.
drp-1 and fis-2 function downstream of, or in parallel to, egl-1 and ced-3, and independently of cps-6 and wah-1.
| Number of extra cells
|
||||
|---|---|---|---|---|
| Genotype | Mean | s.e.m. | Range | n |
| N2 | 10.4 | 0.6 | 7–13 | 15 |
| N2 (heat-shock control) | 10.7 | 0.7 | 7–15 | 15 |
| Phspegl-1 | 40.7 | 2.8 | 25–61 | 15 |
| Phspegl-1; ced-3(n717) | 0.1 | 0 | 0–1 | 15 |
| Phspegl-1; drp-1(tm1108) | 39.7 | 2.3 | 30–64 | 15 |
| Phspegl-1; fis-2(gk363) | 38.7 | 1.6 | 35–51 | 15 |
| Phspegl-1; fis-1(tm1867); fis-2(gk363) | 37.6 | 2.0 | 31–56 | 15 |
| Phspegl-1; drp-1(tm1108); fis-2(gk363) 1 | 24.8 | 2.0 | 13–40 | 15 |
| Pegl-1acCED-3 | 27.7 | 1.1 | 12–41 | 36 |
| Pegl-1acCED-3; drp-1(tm1108) | 27.1 | 0.7 | 18–36 | 33 |
| Pegl-1acCED-3; fis-2(gk363) | 28.2 | 1.2 | 11–37 | 31 |
| Pegl-1acCED-3; drp-1(tm1108); fis-2(gk363) 2 | 19.6 | 1.1 | 7–28 | 34 |
| Pegl-1acCED-3; control(RNAi) | 29.0 | 1.1 | 20–36 | 21 |
| Pegl-1acCED-3; cps-6(RNAi) | 28.5 | 0.8 | 19–35 | 24 |
| Pegl-1acCED-3; wah-1(RNAi) | 27.7 | 1.1 | 17–35 | 23 |
| Pegl-1acCED-3; fis-2(gk363); control(RNAi) | 26.3 | 0.6 | 16–35 | 58 |
| Pegl-1acCED-3; fis-2(gk363); cps-6(RNAi) 3 | 22.3 | 0.9 | 14–31 | 24 |
| Pegl-1acCED-3; fis-2(gk363); wah-1(RNAi) 4 | 23.8 | 0.6 | 12–37 | 77 |
| Pegl-1acCED-3; drp-1(tm1108); fis-2(gk363); control(RNAi) | 21.8 | 0.8 | 13–35 | 47 |
| Pegl-1acCED-3; drp-1(tm1108); fis-2(gk363); cps-6(RNAi) 5 | 17.0 | 0.7 | 11–26 | 33 |
| Pegl-1acCED-3; drp-1(tm1108); fis-2(gk363); wah-1(RNAi) 6 | 16.2 | 0.7 | 9–26 | 30 |
Phspegl-1 (smIs82) is an integrated transgene containing the Phspegl-1 constructs. EGL-1 expression was activated by heat-shock treatment and the number of cell corpses in the head region of 1.5-fold stage embryos was scored. Pegl-1acCED-3 (smIs111) is an integrated transgene containing the Pegl-1acCED-3 construct (Kokel et al., 2006). acCED-3 is therefore expressed in cells normally destined to die. The number of cell corpses was counted in the head region of the 4-fold stage embryos. RNAi experiments were carried out as described in Experimental Procedures. All strains were in the ced-1(e1735); ced-4(n1162) background and confirmed by multiple independent blind test analyses. s.e.m., standard error of the mean. Unpaired two tailed t-test, compared with Phspegl-1 animals:
P = 8.7×10−5; compared with Pegl-1acCED-3 animals:
P=1.2×10−6; compared with control(RNAi) treatment of the same strain:
P=0.00035,
P=0.0063,
P=1.7×10−5,
P=1.5×10−6. Others have P values > 0.05.