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. Author manuscript; available in PMC: 2008 Sep 23.
Published in final edited form as: J Am Chem Soc. 2007 Jun 12;129(26):8072–8073. doi: 10.1021/ja0720816

Figure 4.

Figure 4

Top: Composite image of the separation of TR DHPE and BODIPY DHPE in a POPC bilayer containing 25 mol% cholesterol after 35 minutes of applying a 100 V potential. Bottom: corresponding linescan of the image, corrected for vignetting. The small peak at ~300μm represents an approximately 2% immobile fraction of total dye-labeled molecules. The areas of the large and small peaks of Texas Red are ~ 75% to 25%, which is slightly different than the areas in Figure 1 and probably represents a small amount of batch-to-batch variability in the dye formulation of the manufacturer.