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. 2008 Oct;17(10):1827–1833. doi: 10.1110/ps.034587.108

Figure 2.

Figure 2.

After placement of a SD sequence before the start codon for the plasmid-encoded mutant MjTyrRS, positive selection and negative screen were carried out. These selections were based on the suppression of Asp112TAG in the chloramphenicol acetyltransferase gene. pBR-SD-TyrRS encoded the mutant MjTyrRS that was cotransformed with pAC-tRNA in DH10B cells. (A) Positive selection was carried out in GMML liquid medium with various concentrations of chloramphenicol and p-azido-L-phenylalanine. (B) Negative screen was carried out in GMML liquid medium with various concentrations of chloramphenicol in the absence of p-azido-L-phenylalanine.