Figure 5.

Split Ub measures the proximity between Sec63p and membrane-associated proteins in vivo. Sec63CRUp containing cells expressing N_ub, N_ua, and N_ug constructs of Sec62p (A), Sec61p (B), Ssh1p (C), Bos1p (D), Ste14p (E), Sed5p (F), Sso1p (G), Snc1p (H), Tom22p(I), Vam3p (J), Tpi1p (K), and Guk1p (L) were spotted (10⁵ and 10³ cells) on selective media lacking uracil (A–M) and leucine and histidine (A and D) or leucine and tryptophan (B, C, and E–M) to select for the presence of the C_ub and N_ub constructs. (M) Sec63CRUp-containing cells bearing either the empty plasmid, N_ub-, N_ua-, -N_ug-Sec22p or N_ub-, N_ua-, N_ug-Sec61p were spotted (10⁵, 10⁴, 10³ cells) on plates lacking uracil. Cells were grown for 4 d.