Figure 6.
(A) Nub and Cub constructs of Ste14p are functional. Nub-Ste14p and Ste14CRUp were expressed in cells containing a STE14 deletion and mated with an appropriate tester strain of the opposite mating type. The mated cells were patched on media selecting for the formation of diploids. (B) Ste14p is located between Bos1p and Sed5p. Sec63CRUp containing cells expressing Nvi-Sec62p (a),-Ssh1p (b),-Bos1p (c),-Ste14p (d),-Sed5p (e),-Sso1p (f), and -Snc1p (g) were spotted (105, 104, 103, and 102 cells) on SD-ura plates that also lacked leucine and tryptophan to select for the presence of the Cub and Nvi constructs. Cells were grown for 3 d. (C) Sec62p, Ssh1p, and Sec61p are equidistant to Ste14p. Ste14CRUp-containing cells expressing Nub, Nua, and Nug constructs of Sec62p (a), Ssh1p (b), Sec61p (c), Ste14p (d), Sed5p (e), and Sso1p (f) were spotted (105, 103, and 102 cells) on selective media lacking uracil, leucine, and tryptophan and containing 500 μM methionine to reduce the expression of Ste14CRUp. Cells were grown for 3 d.