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. 1999 Aug;10(8):2519–2530. doi: 10.1091/mbc.10.8.2519

Figure 7.

Figure 7

Tom22p is close to Tom20p; Sso1p and Snc1p are close to Fur4p. (A) Tom20CRUp-containing S. cerevisiae cells expressing the Nub and Nua constructs of Tom22p (a), Sec62p (b), Sso1p (c), and Vam3p (d) were spotted (103 and 102 cells) on selective media lacking uracil. Cells were grown for 3 d. (B) Fur4CRUp containing S. cerevisiae cells expressing the Nub and Nua constructs of Sso1p (a), Snc1p (b), Sec62p (c), and Sed5p (d) were spotted (105 and 103 cells) on selective media lacking uracil. Cells were grown for 3 d. (C) Tom20CRUp-containing cells bearing the UBR1 gene or a UBR1 deletion were transformed with a plasmid harboring Nub-Tom22p or the empty vector pRS314. Cells (103 and 102) were spotted on selective media lacking uracil. Plates were incubated for 3 d.