Table 1.
Growth of cells containing Sec63CRUp and different Nub constructs
| Protein | Nub | Nua | Nug | FOA | Group |
|---|---|---|---|---|---|
| Sec62p | − | − | − | R | 1 |
| Sec61p | − | − | + | R | 2 |
| Sec22p | − | (+) | +++ | R | 3 |
| Ssh1p | − | ++ | +++ | S | 3 |
| Bos1p | − | ++ | +++ | S | 3 |
| Ste14p | − | ++ | +++ | S | 3 |
| Sed5p | (+) | ++ | +++ | S | 3 |
| Sso1p | + | +++ | +++ | S | 4 |
| Snc1p | + | +++ | +++ | S | 4 |
| Tom22p | + | ++ | +++ | ND | 4 |
| Vam3p | +++ | +++ | +++ | S | 5 |
| Tpi1p | +++ | +++ | +++ | S | 5 |
| Guk1p | +++ | +++ | +++ | S | 5 |
Growth was scored on plates lacking uracil. The number of pluses denotes the robustness of the growth of the colonies. The column FOA indicates the behavior of the corresponding Nua construct-bearing cells on plates containing 5-FOA. R, the cells are 5-FOA resistant and grow; S, the cells are 5-FOA sensitive.