Fig. 1.

TyrAG825 decreases erbB2 phosphorylation. A: D54 glioma cells were treated with increasing concentrations (0–50 μM) of TyrAG825. Cell lysates were immunoprecipitated with erbB2-specific antibodies, and then blots were probed with a phosphotyrosine antibody. These blots were then stripped and reprobed with erbB2 as a loading control. Bands on this blot correspond to a molecular weight at ∼190 kD. B: Similarly, when treated cell lysates were immunoprecipitated with a phosphotyrosine antibody and blots probed with erbB2, a similar decrease in tyrosine phosphorylated erbB2 was observed.