Fig. 3.

Biotinylation experiments demonstrate that total and surface expression of BK channels in glioma cells is unchanged. D54 glioma cells were treated with vehicle (serum-free media and 0.1% DMSO), Nrg-1β or TyrAG825 for 12 hr. The cells were biotinylated and lysates collected. The same amount of protein in each treatment condition was incubated with strepavidin beads (surface protein). Blots were probed with an anti-BK antibody and then stripped and reprobed with Na-K-ATPase antibody and an anti-actin antibody to demonstrate surface expression and a lack of intracellular proteins in surface lanes.