Fig. 5.

RT-PCR demonstrates that the gBK insert is present in TyrAG825-treated cells as well as cells in the presence of endogenous neuregulin. A: D54 cells were incubated overnight in normal serum-containing medium (contains endogenous neuregulin), serum-free medium, or serum-free medium with 50 μM TyrAG825. mRNA was collected and RT-PCR performed. Primers were designed to target the specific gBK insert and the four common splicing sites located in the C-terminal tail of the protein shown in B. B: Schematic of the BK channel α-subunit detailing the relative location of each of the four splicing sites targeted with RT-PCR.