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Primer Sets Used for RT-PCR of the C-Terminus of gBK
| Region | Primer sequence | Size (bp) |
|---|---|---|
| Site 1 | 5′ CCCTACTGTTTGTGAGCTGTGTTTTGTGAA 3′ | 202 |
| 5′ CATCATGACAGGCCTTGCAGTAAAAAAAT 3′ | ||
| Site 2/3 | 5′ ATTTTTTTACTGCAAGGCCTGTCATGATG 3′ | 230 |
| 5′ CACCAGTGAAACATCCCAGTAGAGTCGTA 3′ | ||
| Site 4 | 5′ TGTGCGTTATCCTGTCAGCCAATCA 3′ | 275 |
| 5′ CAGGGTCATCATCGTCTTGGTCCA 3′ | ||
| gBK | 5′-GTTGGGAAGAACATTGTGCTTTGTGG-3′ | 150 |
| 5′-ATTTAGGTGACACTATAGAAGTGGACTTTGACAGAGAAAGTTTG-3′ |
