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. Author manuscript; available in PMC: 2008 Sep 23.
Published in final edited form as: Glia. 2004 May;46(4):419–436. doi: 10.1002/glia.10361

Fig. 4.

Fig. 4

Chloride channel blockers inhibit the hypotonically-activated chloride channel in astrocytes. A–C: Representative whole-cell recordings of NPPB-sensitive (A), NA-sensitive (B), and DIDS-sensitive (C) currents obtained from subtraction of current traces in response to voltage steps from -80 mV to +80 mV from a holding potential of 0 mV (inset) in ChCl hypotonic bath and ChCl pipette solutions before and after NPPB, NA or DIDS application, respectively. D,E: Percentage current block at +60 mV (D) and -60 mV (E) for different chloride channel blockers of interest. Values were obtained by comparison of current amplitudes in response to voltage steps following 2-min hypotonic challenge only and in the presence of the blocker of interest. Note that negative current block was obtained for control cells, as the current amplitudes continued to increase with time in hypotonic challenge. Asterisks indicate a statistically significant (*P < 0.05, **P < 0.001) change from control amplitudes.