The transport-mediated component of volume regulation can be inhibited at higher temperatures than the channel-mediated component. A: normalized mean cell volumes of D54-MG cells exposed to a 50% addition of ddH2O at 4, 10, 15, 25, and 37°C. B: normalized mean cell volumes of D54-MG cells exposed to a 50% addition of ddH2O while maintaining the temperature of the experiment at 15°C. Cells were treated with DIOA, NPPB + Cd2+, or no drug (control).