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. Author manuscript; available in PMC: 2008 Sep 23.
Published in final edited form as: J Biol Chem. 1999 Apr 16;274(16):11139–11149. doi: 10.1074/jbc.274.16.11139

Fig. 2. Presence of a PtdIns-dependent phosphotransferase in membranes of R. leguminosarum.

Fig. 2

Membranes of different strains were assayed for phosphotransferase activity using the standard assay. A protein concentration of 0.2 mg/ml was used, and the incubation was carried out for 20 min at 30 °C. The thin layer chromatographic analysis of the reaction products generated from Kdo2-[4′-32P]lipid IVA and PtdIns are shown. Lane 1, no membranes; lane 2, E. coli W3110; lane 3, R. leguminosarum biovar etli CE3; lane 4, R. leguminosarum biovar trifolii 24AR; lane 5, R. leguminosarum biovar viciae 8401; lane 6, R. leguminosarum biovar trifolii ATCC 14479; lane 7, R. meliloti 1021. No PtdIns-4-P was formed in the absence of added PtdIns.