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. Author manuscript; available in PMC: 2008 Sep 23.
Published in final edited form as: J Biol Chem. 1999 Apr 16;274(16):11139–11149. doi: 10.1074/jbc.274.16.11139

Fig. 3. Inner membrane localization of the 4′-phosphatase and the PtdIns-dependent phosphotransferase of R. leguminosarum.

Fig. 3

The inner and outer membranes of strain CE3 were separated by isopycnic sucrose density gradient centrifugation, and ~0.4-ml fractions were collected. NADH oxidase and phospholipase A activities were assayed to locate inner and outer membrane fragments, respectively. A, NADH oxidase and phospholipase A activities in each fraction are expressed as a percentage of the total activity across the entire gradient. B, the 4′-phosphatase and the phosphotransferase activities were assayed in each fraction.