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. Author manuscript; available in PMC: 2008 Sep 23.
Published in final edited form as: J Biol Chem. 1999 Apr 16;274(16):11139–11149. doi: 10.1074/jbc.274.16.11139

Fig. 6. Thermal inactivation of the 4′-phosphatase and the phosphotransferase.

Fig. 6

Samples of the partially purified enzyme (heparin-agarose step) were preincubated at various temperatures (30, 37, or 45 °C) for the indicated times. A portion of the incubation was then used to assay for remaining 4′-phosphatase and phosphotransferase activities. The percentage of the activity remaining is normalized to a control sample of the enzyme held on ice.