Fig. 9. Acceptor substrate specificity of the phosphotransferase for PtdIns versus PtdGro.
The effects of PtdIns and PtdGro concentrations on phosphotransferase specific activity were measured in the presence of excess of Kdo2-[4′-32P]lipid IVA (50 μM). Heparin-agarose-purified enzyme (10 μg/ml) was used, and the incubation was carried out for 10 min at 30 °C.