| Cycle number | Denature | Anneal | Extend |
|---|---|---|---|
| 1 | 95 °C 2:00 min | — | — |
| 2−41 | 95 °C 0:40 min | 58 °C 0:40 min | 72 °C 1:00 min |
| 42 | — | — | 72 °C 5:00 min |
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