| Component | Amount | Final concentration |
|---|---|---|
| Liver DNA | 100 ng | 2 ng μl−1 (≈ 30,000 haploid genomes) |
| EP-F1 and EP-R1 primers | 10 pmol each | 200 nM |
| dNTPs (A, G, C, T) | 10 nmol each | 200 μM |
| 5× Optimized Buffer A | 10 μl | 1× Optimized Buffer A |
| DMSO | 2.5 μl 100% stock | 5% (vol/vol) |
| Glycerol | 2.5 μl 100% stock | 5% (vol/vol) |
| Taq DNA polymerase | 5 U | 0.1 U μl−1 |
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