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. Author manuscript; available in PMC: 2008 Sep 23.
Published in final edited form as: Int J Cancer. 2008 Sep 15;123(6):1318–1326. doi: 10.1002/ijc.23674

Figure 2.

Figure 2

Effects of siRNA on FAAH expression, hydrolysis of AEA and 2-OG, and invasion of LNCaP cells. A) Western blot depicting immunoreactive bands corresponding to FAAH in control, siControl, and FAAH siRNA in membrane fractions of LNCaP cells. The intensity of immunoreactive bands was normalized to the intensity of β-actin (labeled as intensity ratio). B) Specific hydrolysis activity of AEA and 2-OG in membrane fractions in non-transfected (control), siControl, and FAAH siRNA in LNCaP cell membrane fractions. Values are mean ± S.E.M. (n = 6–8). #, significantly lower than control, p < 0.001; *, significantly lower than control p < 0.05. C) Invasion of LNCaP cells in non-transfected (control), siControl, and FAAH siRNA-transfected cells, and FAAH siRNA-transfected cells treated with 2-AG (10 μM) in the absence and presence of SR-141716A (500 nM). Values are mean ± S.E.M. (n = 6–12). *, significantly lower than control, p < 0.05. **, significantly lower than FAAH siRNA-transfected cells, p < 0.05. #, significantly higher than FAAH siRNA-transfected cells treated with 2-AG, p < 0.05. D) Invasion of LNCaP cells in non-transfected (control), siControl, and FAAH siRNA-transfected cells, and FAAH siRNA-transfected cells treated with AEA (1 μM) in the absence and presence SR-141716A (500 nM). Values are mean ± S.E.M. (n = 6–12). *, significantly lower than control, p < 0.05. **, significantly lower than FAAH siRNA-transfected cells, p < 0.05. #, significantly higher than FAAH siRNA-transfected cells treated with AEA, p < 0.05.