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. 1985 Jun;54(3):661–664. doi: 10.1128/jvi.54.3.661-664.1985

Intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides.

S L Wechsler, D M Lambert, M S Galinski, M W Pons
PMCID: PMC254849  PMID: 2987519

Abstract

The intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides was examined by polyacrylamide gel electrophoresis of [35S]methionine-labeled cell extracts under reducing conditions. All of the virion structural proteins were detected in cell extracts, including: L, 180,000 molecular weight (180K); P, 83K; HN, 69K; NP, 66K; F0, 60K; F1, 51K; and M, 38K. P and NP were phosphorylated. HN and F were glycosylated. The kinetics of intracellular viral protein synthesis did not detect any early or late proteins. Pulse-chase experiments failed to detect any precursor-product relationships. No nonstructural proteins were detected.

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Selected References

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