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Overexpression of phospholipase C suppresses the toxicity of the TOR1 toxic domain. Yeast strain CAY6 was cotransformed with plasmids expressing the TOR1-toxic domains (AA 1207–1961 in panel A and AA 1207–2340 in panel B), and plasmids overexpressing the STT4 (PGALHA-STT4), MSS4 (pGMSS4), and PLC1 (pJF137), proteins, respectively, under the control of the galactose promoter, were grown in minimal medium containing glucose or galactose for 4 d at 30°C.
