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Binding of Cy3-[Sar9 MetO211]-SP to KNRK-NK1-R cells (A–C) or untransfected KNRK cells (D). Cells were incubated with 190 nM Cy3-[Sar9 MetO211]-SP for 1 h at 4°C, fixed, and observed. (A) KNRK-NK1-R cells. (B) KNRK-NK1-R cells preincubated with 1 μM unlabeled [Sar9 MetO211]-SP before addition of Cy3-[Sar9 MetO211]-SP. (C) KNRK-NK1-R cells preincubated with 1 μM CP96345, an NK1-R–selective antagonist, before addition of Cy3-[Sar9 MetO211]-SP. (D) KNRK cells. Bar, 10 μm.
