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Characterization of antibodies by Western blotting. Extracts of myenteric neurons (50 μg protein/lane for Gαq/11, β-arrestin-1 and -2, and GRK-2; 25 μg protein/lane for GRK-2 and -3) were separated on 12% SDS-PAGE gels and probed with antibodies to Gαq/11, β-arrestin-1 and -2, GRK-2, and GRK-2 and -3.
