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Effects of graded concentrations of SP on Ca2+ mobilization in myenteric neurons. Neurons were exposed to a single concentration of SP for 1 min, and the maximal increase in the 340:380 nm fluorescence ratio (A) and the number of responsive neurons per field (B) were measured. Results in A are the mean ± SE of measurements from n = 133 neurons. Results in B are the mean ± SE of measurements from n = 3 coverslips. Different neurons were used for each SP concentration tested.
