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. 1999 Aug;10(8):2559–2572. doi: 10.1091/mbc.10.8.2559

Figure 2.

Figure 2

Activation of a yeast pheromone-inducible gene by a Schizophyllum pheromone–receptor pair. Cell culture supernatants were collected from MATa strains (W303–1A) containing pPGK-bbp2(4), which encodes the Schizophyllum pheromone Bbp2(4) (black), or the control vector pPGK (stripes). Response to culture supernatants or a fresh medium control (white) was tested using a MATα ste3 strain (SDK45) containing the pheromone-inducible FUS1-lacZ reporter plasmid and either pPGK-bbr1, which encodes Schizophyllum receptor Bbr1, or pPGK. β-Galactosidase activity was assayed to assess pheromone response. Error bars here and in subsequent figures represent 1 SD.