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. 1999 Aug;10(8):2559–2572. doi: 10.1091/mbc.10.8.2559

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Copyright © 1999, The American Society for Cell Biology

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Bbp2(4) processing and secretion are independent of transporter Ste6p and all other a-specific gene products. The MATα ste3 strain (SDK45) containing the pheromone-inducible FUS1-lacZ reporter plasmid and pPGK-bbr1 was tested for response. Reporter gene assays were done as in Figure 2. (A) The effects of cell culture supernatants from the MATa STE6 strain (W303–1A) and the isogenic ste6 strain (RAK32) containing either pPGK (stripes) or pPGK-bbp2(4) (black) were compared. (B) The effects of cell culture supernatants from MATa (W303–1A) and MATα (W303–1B) cells containing either pPGK (stripes) or pPGK-bbp2(4) (black) were compared.