Table 2.
Oligonucleotides
| Gene | Oligonucleotide | Position | Sequencea |
|---|---|---|---|
| bbr1 | 980420-3 | Upstream | TTCGAATTCATGCACCCCGAGTTTGCCCCCGTAG |
| 980420-4 | Downstream | TCCGGATCCCTACCTGCGCCCACCGGGGAACAC | |
| bbr2 | 980730-1 | Upstream | TTCGAATTCATGTACTCCAACGACCCGACG |
| 980730-2 | Downstream | TCCGGATCCTTAAAGGACGACGCGGTGTG | |
| bbp1(1) | 980730-5 | Upstream | TTCGAATTCATGGACGCCTTCACCGCC |
| 980730-6 | Downstream | TCCGGATCCCTACGCAACCACGCACCAC | |
| bbp2(4) | 980227-2 | Upstream | CGGAATTCATGGACGACTTCATCACC |
| 980227-3 | Downstream | CGGGATCCGGCTACGCCGGGTCACGCC |
a
The oligonucleotide sequences are shown in the 5′ to 3′ direction. Single underlines indicate EcoRI sites in the upstream primers or BamHI sites in the downstream primers. Double underlines indicate an ATG initiation codon in upstream primers or a termination codon in the reverse strand of downstream primers; 980730-2 binds downstream of the termination codon.