Figure 3.

Binding of GST-Act3p(Ins II) to purified core histones. (A) Fragments used for the construction of the GST fusions. Numbers correspond to the N- and C-terminal amino acids retained of each fragment. Chicken histones H3 and H4 (H3/4 in B), chicken histones H2A and H2B (H2A/B in B), and acid-precipitated yeast core histones (H2A/B/3/4 in C) were electrophoresed on SDS-polyacrylamide gels. The gels were subjected to staining with Coomassie brilliant blue R-250 or to far Western blot analyses with ³²P-GST-Act3p(Ins II), ³²P-GST-Act3p(N-ter), ³²P-GST-Act3p(C-ter), and ³²P-GST-Act1p(flank). Panels for the far Western blot analyses with ³²P-GST-Act3p(Ins II) differ in the time of autoradiographic exposure.