Figure 6.
Immunoprecipitation of protein complex(es) with anti-histone H2A monoclonal antibody. Protein complex(es) expected to contain Act3p-His6 in the extract from strain MZ3ACT3H were enriched with a Ni2+-NTA column. Aliquots of the fraction eluted with a buffer containing 0.3 M imidazole were subjected to immunoprecipitation using anti-histone H2A antibody. The presence of Act3p in the input fraction (lane 1) and in the fraction immunoprecipitated with anti-histone H2A antibody (lane 2) or with a serum from a nonimmunized mouse (lane 3) was examined by Western blot analysis with anti-Act3p antiserum. Positions for Act3p are marked on the left.