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The association of Mad1p and Mad2p is independent of Mad3p, Bub1p, Bub2p, and Bub3p. Cell lysates or Mad1p immunoprecipitates prepared from nocodazole-treated wild-type (WT) or mutant strains, as indicated on top, were immunoblotted with an anti-Mad1p (upper panel) or an anti-Mad2p (lower panel) antibody. All mad or bub mutant strains were deletions, except for bub2-1.
