Figure 6.

wis4⁺ does not suppress the win1-1 defect under high-osmolarity conditions. The win1-1 strain carrying multiple copies of a genomic fragment containing the wis4⁺ gene was compared with the wild-type strain. (A) Cells in a colony were photographed after 3 d on EMM+ 1.2 M KCl or 2 d on EMM (control). (B) The phosphotyrosine content of Spc1 in wild-type or win1-1 strains determined by anti-phosphotyrosine antibody. The HA blot shows the relative amounts of HA-tagged Spc1. Cells were collected by filtration before or 10 min after exposure to 0.6 M KCl.