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. 1999 Aug;10(8):2631–2645. doi: 10.1091/mbc.10.8.2631

Figure 4.

Figure 4

ADP binding induces a conformational change in HsCdc6. (A) GST-HsCdc6 (0.5 μg) bound to glutathione-agarose was partially digested with trypsin in the absence of nucleotide (lane 3) or in the presence of 2 mM ATP (lane 4), ATPγS (lane 5), ADP (lane 6), or UTP (lane 7). The reaction was analyzed by SDS-PAGE (15% polyacrylamide gel) and silver staining. No trypsin was added to the reaction shown in lane 2. (B and C) The experiment in A was repeated, except that GST-HsCdc6 (K208A) (B) or GST-HsCdc6 (E285Q) (C) was used instead of wild-type protein. M, 10-kDa marker protein ladder.