Figure 1.

(A) Transcription in permeabilized cells from two wild-type lymphoblastoid cells (SNW646 and GM1310B) and two WS lymphoblastoid cells (JO1050 and KO375). The cells in exponential growth were prelabeled with [¹⁴C]thymidine and permeabilized with lysolecithin (20 μg/ml), and transcription was carried out at 37°C. The radioactivity incorporated was determined by scintillation counting and expressed as [³H]UMP incorporated per 10⁶ cells. Error bars indicate the SEM of six independent experiments. (B) Comparison of the rate of transcription in wild-type and WS lymphoblastoid cell lines. The cells were prelabeled with [¹⁴C]thymidine, encapsulated, and lysed with lysolecithin, and transcription was carried out for 30 min at 37°C. Transcription levels for each cell line were normalized to the level for the wild-type (non-WS) SNW646 cell line.