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. 1999 Aug;10(8):2655–2668. doi: 10.1091/mbc.10.8.2655

Figure 4.

Figure 4

In vitro RNA pol II transcription from the AdML promoter in extracts of wild-type and WS cells. (A) Transcription from the AdML promoter carried on a supercoiled plasmid with a G-less cassette was assayed using nuclear extracts from wild-type (SNW 646) and WS (JO1050 and KO375) lymphoblasts. Incubation of plasmid DNA was carried out with nuclear extracts (75 μg of total protein) for 1 h, and transcription was monitored by electrophoresis and phosphorimaging (see MATERIALS AND METHODS for details). RNA pol II-specific transcription is evidenced by the production of two transcripts ∼400 nucleotides in length. (B) Transcription using the nuclear extracts from wild-type (SNW646) and WS(KO375) cells was assayed over a range of extract concentrations (20, 40, and 80 μg of protein). (C) Relative levels of transcription in WS nuclear extracts. Transcription was measured using multiple independently prepared nuclear extracts and normalized to the transcription levels using nuclear extracts from a wild-type cell line (SNW646). The error bars represent the SEM between individual experiments.