Figure 9.

Immunofluorescent staining of WRN helicase in the nucleoplasm of WS (AG11395) and normal fibroblasts (GM38A). Exponentially growing normal human and WS fibroblasts were fixed in acetone:methanol. Cells were stained with a rabbit polyclonal antibody to WRNp and a Texas Red–conjugated secondary antibody to rabbit immunoglobulin G. The red dots indicate the positions of WRNp in the nucleoplasm. WS fibroblasts did not show any staining (A), whereas the normal cells showed distinct staining of WRNp (B). (C) Colocalization of both nucleolar (green fluorescence) and WRNp (red fluorescence) in the interphase nuclei of GM38 cells detected with fluorescein- and Texas Red–conjugated secondary antibodies.