Table 2.
Cell line | Transcription (%)
|
|
---|---|---|
15 min | 30 min | |
GM1310B chromatina | 100 | 100 |
KO375 chromatin | 37.4 | 32.8 |
KO375 chromatin + Hela nuclear extract | 116 | 103 |
KO375 chromatin + KO375 nuclear extract | 39.7 | 30.9 |
The transcription measured as [3H]UMP incorporated per 106 cells in the normal cell line (GM1310) was considered 100%. The transcription measured in WS (KO375) chromatin supplemented with 50 μg of either Hela or KO375 nuclear extracts was expressed as percent of control.