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. 1998 Sep;9(9):2337–2347. doi: 10.1091/mbc.9.9.2337

Figure 4.

Figure 4

Western blots of WT and oda cytoplasmic extracts test subunit stability. Cytoplasmic extracts of WT and oda1-oda11 prepared as in Figure 2 were probed with mAbs C11.6 (anti-HCβ), 25–8 (anti-HCγ), 1878A (anti-IC78), and 1869A (anti-IC70). (A) Outer dynein arm proteins (indicated along the right margin) present in cytoplasmic extracts of WT and oda1–11. Most oda mutant samples display antigen levels similar to WT, but there are several exceptions as discussed in text. Multiple bands below HCβ in WT, oda1, and oda3 are due to the breakdown of HCβ. No detectable HCβ was found in oda4, no HCγ was found in oda2, no IC78 was found in oda9, and no IC70 was found in oda6 (at this exposure; see text and Figure 3C). 1878A (anti-IC70) cross-reacted with cytoplasm proteins migrating faster than 78 kDa (asterisk) that are apparently unrelated to flagellar dynein. (B) Western blots of a second set of independently prepared cytoplasmic extracts from oda5-oda10 probed with 1878A (anti-IC78) and 1869A (anti-IC70) that illustrate the variability in the reductions of IC70 in oda9 and IC78 in oda6 (compare with bottom two panels in panel A). (C) Upper and lower panels show the results of a 10-fold increase in exposure time for lanes oda5–oda7 from the bottom panels of Figure 3A and Figure 3B, respectively, and reveal an IC70 band in oda6 cytoplasmic extracts.