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α- and β-Tubulin coimmunoprecipitate with low efficiency from tub1-724 cells. Immunoblots with anti-α-tubulin (top row) and anti-β-tubulin (bottom row) of whole-cell extracts (WCE) and the precipitates with the two antibodies (αIP and βIP) from wild-type TUB1 or mutant tub1-724 cells.