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Overexpression of PAC2 is lethal in TUB1/tub1-724 heterozygous cells. Serial (fourfold) dilutions of saturated cultures were plated to galactose-containing media and allowed to grow at 30°C. Strains were wild-type diploids or TUB1/tub1-724 cells containing either YCpGAL or CEN-GAL-PAC2.
