Table 1.
Strains and plasmids
| Strain/plasmid | Genotype | Reference |
|---|---|---|
| Strains | ||
| FSY185 | a/α; ura3-52/ura3-52, leu2-3,112/leu2-3,112, his3Δ200/his3Δ200, lys2-801/lys2-801, ade2/ADE2 | Weinstein and Solomon, 1990 |
| FSY183 | a; ura3-52; leu2-3,112; his3Δ200; lys2-801 | Weinstein and Solomon, 1990 |
| FSY157 | a; ura3-52; leu2-3,112; his3Δ200; lys2-801; Δtub1::HIS3, Δtub3::TRP1 [pRB624] | Schatz et al., 1988 |
| FSY182 | a; ura3-52; leu2-3,112; his3Δ200; lys2-801; Δtub1::HIS3, Δtub3::TRP1 [pRB539] | Schatz et al., 1988 |
| LTY8 | FSY157 plus YCpGAL | Archer et al., 1995 |
| LTY11 | FSY182 plus YCpGAL | This study |
| LTY291 | FSY157 plus pGHR | This study |
| LTY292 | FSY182 plus pGHR | This study |
| LTY374 | FSY157 plus pPA45 | This study |
| LTY376 | FSY182 plus pPA45 | This study |
| JAY47 | a/α, ura3-52/ura3-52, leu2-3,112/leu2-3,112, his3Δ200/his3Δ200, lys2-801/lys2-801, ade2/ADE2, TUB2/TUB2-LEU2-GAL-TUB2 | Archer et al., 1995 |
| LTY319 | JAY47 plus YCp50 | This study |
| LTY321 | JAY47 plus A21A | This study |
| LTY323 | JAY47 plus pLV32 | This study |
| LTY325 | JAY47 plus pA1A5106 | This study |
| LTY338 | JAY47 plus pLV30 | This study |
| LTY340 | JAY47 plus pLV38 | This study |
| LTY343 | JAY47 plus pLV36 | This study |
| LTY345 | JAY47 plus pLV37 | This study |
| LTY392 | a/α; ura3-52/ura3-52; leu2-3,112/leu2-3,112; his3Δ200/his3Δ200; lys2-801/lys2-801; TUB1/Δtub1::HIS3, TUB3/Δtub3::TRP1; [pRB539, pA5] | This study |
| LTY393 | like LTY392 but with pPA45 rather than pA5 | This study |
| LTY395 | like LTY392 but with YCpGAL rather than pA5 | This study |
| LTY396 | a/α; ura3-52/ura3-52; leu2-3,112/leu2-3,112; his3Δ200/his3Δ200; lys2-801/lys2-801; TUB1/Δtub1::HIS3,TUB3/Δtub3::TRP1, [pRB624, pPA45] | This study |
| LTY397 | like LTY396 but with pA5 rather than pA45 | This study |
| LTY440 | JAY47 plus YCpGAL | This study |
| LTY439 | JAY47 plus pLV56 | This study |
| LTY399 | like LTY396 but with YCpGAL rather than pA45 | This study |
| LTY539 | FSY183 plus pJA3 and pLV62 | This study |
| LTY541 | FSY183 plus pJA3 and pRS317 | This study |
| LTY540 | FSY183 plus YCpGAL and pLV62 | This study |
| Plasmids | ||
| YCp50 | CEN-URA3 | Kirkpatrick and Solomon, 1994 |
| pA1A5106 | TUB1-CEN-URA3 | Kirkpatrick and Solomon, 1994 |
| pA21A | RBL2-CEN-URA3 | Archer et al., 1995 |
| pA5 | GAL 1-10-RBL2-URA3 | Archer et al., 1995 |
| pGHR | GAL 1-10-HIS6-RBL2-URA3 | Archer et al., 1998 |
| pRB624 | tub1-724-CEN-LEU2 | Schatz et al., 1988 |
| pRB539 | TUB1-CEN-LEU2 | Schatz et al., 1988 |
| pPA45 | GAL 1-10-PAC2-CEN-URA3 | Alvarez et al., 1998 |
| YCpGAL | GAL1-10-CEN-URA3 | Archer et al., 1995 |
| pLV30 | tub1-704 in YCp50 | This study |
| pLV32 | tub1-724 in YCp50 | This study |
| pLV36 | tub1-737 in YCp50 | This study |
| pLV37 | tub1-747 in YCp50 | This study |
| pLV38 | tub1-714 in YCp50 | This study |
| pLV56 | GAL 1-10-PAC2-HA-HIS6-CEN-URA3 | This study |
| pLV62 | GAL1-10-PAC2-HA-HIS6-CEN-LYS2 | This study |
| pRS317 | CEN-LYS2 | Sikorski and Hieter, 1989 |