Table 2.
Rescue of excess β-tubulin lethality by α-tubulin alleles
| Plasmid | 30°C | 18°C |
|---|---|---|
| .04 | .03 | |
| RBL2 | 6.8 | 7.2 |
| TUB1 | 12.0 | 15.4 |
| tub1-724 | .84 | .06 |
| tub1-704 | 10.9 | 18.9 |
| −714 | 14.0 | 20.0 |
| −737 | 4.1 | 8.5 |
| −747 | 11.8 | 21.0 |
JAY47 cells, which contain an integrated GAL-TUB2 gene, carrying the indicated α-tubulin alleles on plasmids were plated to media containing either galactose or glucose and incubated at either 30 or 18°C. Rescue is reported as the percentage of cells that form colonies on galactose plates compared with glucose plates.