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. 1998 Sep;9(9):2407–2422. doi: 10.1091/mbc.9.9.2407

Figure 6.

Figure 6

The 3′-ETS pre-rRNA is present in the NDF of CMT3 cells. The 3′-ETS pre-rRNA segment was detected by fluorescence in situ hybridization with a biotinylated antisense riboprobe (nucleotides +13,111/+13,343). (A–C) Labeling of interphase cells with the 3′-ETS probe and the anti-B23 antibody. The 3′-ETS signal was present in restricted intranucleolar areas (A) in contrast to general nucleolar staining with anti-B23 antibody as seen in panel B and in the overlay (C). (D and E) In anaphase cells the 3′-ETS signal is detectable throughout the cytoplasm with several brighter foci (D, small arrows) that were identified as the NDF by colocalization with protein B23 (E, small arrows). (F and G) In late telophase cells the 3′-ETS signal reappeared in the reformed nucleoli (F, arrowheads), but the signal is also present in persisting cytoplasmic NDF (F, small arrows) where it colocalizes with protein B23 (G, small arrows). Bar, 10 μm.