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BoNT-C1 dramatically reduces syntaxin vesicle localization. Cells treated with 3.9 and 5.3 nM BoNT-C1 have an ∼30 and 70% decrease in syntaxin immunolocalization, respectively, when compared with untreated cells of the same embryo, whereas rab3 immunolocalization is unaffected by treatment with the toxin. Each bar represents the average relative immunolocalization of 15 confocal sections of three different embryos.
