Figure 11.

Immunolabeling of VAMP5 in young and mature C2C12 myotube cultures. C2C12 cells were grown in culture snd studied at either day 3 (a) or day 9 (b–d) after differentiation. Cells were fixed and labeled with anti-VAMP5 antibodies. In young cultures, both intracellular and surface labelings were evident. In mature cultures, surface labeling became more predominant. Panels b–d represent sequential serial sections using a confocal laser microscope through the same field of a mature culture and illustrate the relative absence of intracellular labeling compared with that observed in panel a. Representative nuclei in both preparations are indicated (n). Bar, 10 μm.