Figure 8.

Differentiated C2C12 cells were double-labeled with rabbit antibodies against VAMP5 and a mouse monoclonal antibody against GS28 and then with FITC-conjugated anti-rabbit IgG and rhodamine-conjugated anti-mouse IgG. Cells were viewed with confocal microscopy, and combined images are shown. In myotubes, both surface and intracellular labelings are detected, and VAMP5 is thus associated with the plasma membrane and intracellular vesicular structures of myotubes. The broken-ring labeling of VAMP5 around the nuclei overlaps well with that of GS28, as does some of the peripheral labeling. However, the labeling on the plasma membrane and the majority of peripheral vesicular structures labeled by VAMP5 are devoid of GS28 As shown, most unfused myoblasts (except for the one on the right bottom corners of a–c) do not exhibit specific labeling of VAMP5, although their Golgi apparatuses are labeled by the GS28 monoclonal antibody. Bar, 10 μm.