Figure 10.

NPC/NE association of Nup82p, a GLFG nucleoporin(s), and Nup159p are not markedly diminished in nup57-E17 cells. Direct fluorescence microscopy was performed on wt (SWY1411) and nup57-E17 (SWY1744) strains expressing GFP-tagged Nup82p (left column). Indirect immunofluorescence microscopy was performed on wt (SWY519) and nup57-E17 (SWY1587) strains using antibodies recognizing either the GLFG family of nucleoporins (middle column) or Nup159p (right column), with DAPI staining shown for the anti-Nup159p fields. Cells were shifted to growth at 37°C for 4 h. Within each column, images were photographed and printed for identical times for direct comparison of fluorescence intensities.