Figure 11.

Localization of the GLFG nucleoporin Nup116p at the NPC/NE is perturbed in temperature-arrested nup57-E17 cells, whereas Nup145-Cp remains predominantly at the NPC/NE. Indirect immunofluorescence microscopy was performed on wt (SWY519) and nup57-E17 (SWY1587) strains using antibodies recognizing either the C-terminal region of Nup145p (left column) or the C-terminal region of Nup116p (middle column). Coincident DAPI staining for the anti-Nup116p field is shown. Cells were shifted to growth at 37°C for 4 h. Within each column, images were photographed and printed for identical times for direct comparison of fluorescence intensities.